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b16ova in house  (ATCC)


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    ATCC b16ova in house
    B16ova In House, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b16ova+in+house/B16-F10/pm36933554-253-169-197
    Average 99 stars, based on 7917 article reviews
    b16ova in house - by Bioz Stars, 2026-09
    99/100 stars

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    Membrane:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2

    SYBR Green Assay:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2

    Cell Isolation:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2

    Plasmid Preparation:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2

    Enzyme-linked Immunosorbent Assay:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2

    Transgenic Assay:

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2 Article ll OPEN ACCESS

    Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mitochondrial Membrane Potential Kit (TMRM) Merck Cat#MAK159-1KT hgp100 (25–33) peptide Genscript Cat#RP20344 OVA (257–264) peptide Invivogen Cat#vac-sin DNAse I Merck Cat#1128493001 HindIII digestion enzyme New England Biolabs Cat#R0104S Benzyl-a-GalNAc Sigma-Aldrich Cat#200100 2-NBDG Cayman Cat# 186689-07-6 Collagenase-D Roche Cat#11088866001 Ficoll Paque Fisher Scientific Cat#17144003 Lectin kit I-Fluorescein Vector labs Cat# FLK-2100 Lectin kit II-Biotinylated Vector labs Cat# BK-2100 AAL lectin-Biotinylated Vector labs Cat# B-1395-1 Protamine-sulfate Sigma-Aldrich Cat#P4020 M-MLV Invitrogen Cat#28025-013 iQTM SYBR Green Supermix BIO-RAD Cat#170-8882 Ringer Lactado Grifols Cat#637066 Critical commercial assays Mouse CD8+ T cell isolation kit Miltenyi Biotech Cat#130-104-075 T7 mScriptTM Standard mRNA Production System CellScript Cat#C-MSC100625 RNeasy Micro Kit Qiagen Cat#74004 Endofree plasmid maxi kit Qiagen Cat#50912362 mouse IFNg OptEIA set BD Biosciences Cat#551866 Mouse IL-12 (p70) ELISA set BD Biosciences Cat#555256 ELISA Mouse Duoset IL22 R and D Systems Cat#DY582-05 Mouse ProcartaPlex Mix&Match 13-plex Thermofisher Cat#PPX-13MX2W9XU Maxwell RSC simplyRNA tissue Promega Cat#AS1340 Deposited data RNAseq data This paper GEO: GSE206195 Experimental models: Cell lines Mouse: MC38 In house N/A Mouse: B16OVA In house (Weigelin et al., 2015)85 N/A Mouse B16F10 In house (Weigelin et al., 2015)85 N/A Mouse: B16OVAgp75 In house (Weigelin et al., 2015)85 N/A Mouse: MS-1 ATCC RRID:CVCL_6502 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo Strain: C57BL/6JOlaHsd Mouse Pmel1 TCR transgenic Jackson Laboratory Strain: 005023 Mouse: BATF3 / on C57BL/6 background In house N/A Mouse: CD45.1 on C57BL/6 background In house N/A Mouse: OT-I TCR transgenic Jackson Laboratory Strain: 003831 Oligonucleotides Mouse single-chain IL-12 mRNA In house (Etxeberria et al., 2019)29 N/A Mouse IL-18 mRNA This paper N/A Mouse DRIL18 mRNA (Zhou et al., 2020)26 N/A Mir155 mouse primers This paper N/A Inpp5d (SHIP) mouse primers This paper N/A (Continued on next page) Cell Reports Medicine 4, 100978, March 21, 2023 e2



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